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KMID : 1098420170250060367
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Korean Journal of Medicinal Crop Science
2017 Volume.25 No. 6 p.367 ~ p.374
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Anti-obesity Effects of Barley Sprout Young Leaf on 3T3-L1 Cells and High-fat Diet-induced Obese Mice
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Kang Byoung-Man
Sim Mi-Ok
Kim Min-Suk
Yoo Seung-Jin
Yeo Jun-Hwan
Jung Won-Seok
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Abstract
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Background: An imbalance in energy intake and expenditure can cause obesity, which is a major risk factor for chronic diseases such as heart disease, type 2 diabetes, insulin resistance, cancers and hyperlipidemia.
Methods and Results: In this study, we evaluated the anti-obesity effects of a water extract from the young leaves of barley sprout (BS) in 3T3-L1 cells and in high-fat diet (HFD)-induced obese mice (HF). Lipid accumulation measurement indicates that BS markedly inhibited adipogenesis by reducing lipid droplet production in a dose-dependent manner. Furthermore, the mRNA expression of adipogenic transcription factors peroxisome proliferator-activated receptor-γ and fatty acid synthetase, CCAAT/enhancer binding protein-α and fatty acid binding protein 4 in 3T3-L1 cells was significantly inhibited by BS treatment. In an in vivo test, the BSadministered group of HFD-induced mice showed less body weight gain, and lower liver and epididymal white adipose tissue weights. The BS-treated mice showed decreased serum levels of leptin and lipids compared to untreated HFD mice and the levels of adiponectin and the HDL-cholesterol/total cholesterol ratio increased. These results indicate that BS inhibits body fat accumulation by reducing the mRNA expression of lipogenesis transcription factors and increasing serum adipokine concentration in in vitro and in vivo tests.
Conclusions: BS reduced high fat diet-induced weight gain and had a positive effect on dyslipidemia.
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KEYWORD
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Hordeum vulgare L., Adipogenesis, C/EBPα, FAS, Lipogenesis, PPARγ, C/EBPα
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